ITGA2 Antikörper

Produktnummer ABIN118720

Dieser Maus Monoklonal Antikörper erkennt spezifisch ITGA2 in FACS, WB, IP, IHC (p), IHC (fro) und EIA. Er zeigt eine Reaktivität gegenüber Human und Affe.

Kurzübersicht für ITGA2 Antikörper (ABIN118720)

Target: ITGA2 (Integrin alpha 2 (ITGA2))

Reaktivität: Human, Affe

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ITGA2 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Klon: 16B4

Produktdetails anti-ITGA2 Antikörper

Spezifität: This antibody is identified as capable of immunoprecipitating a non-reducible alpha subunit from I125 surface labelled cell line extract. It is confirmed as specific to the alpha 2 subunit by relative expression of antigen on various cell lines by FACS, and its recognition of affinity purified alpha 2 beta 1 in dot blots.

Kreuzreaktivität (Details): Species reactivity (tested):Human.

Aufreinigung: Affinity Chromatography on Protein G

Immunogen: Purified human beta1 preparation from HT1080 fibrosarcoma cell extract. Spleen cells from immunised BALB/c mice were fused with cells of the X63/Ag8.653 mouse myeloma cell line.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Flow Cytometry: 30 μg/mL, Immunoprecipitation: 25-50 μg/mL. Immunohistochemistry on Frozen Sections: 30-40 μg/mL. ELISA: 10 μg/mL. Western Blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu ITGA2

Target: ITGA2 (Integrin alpha 2 (ITGA2))

Andere Bezeichnung: CD49b / ITGA2

Hintergrund: CD49b, a member of the integrin family of extracellular matrix and cell adhesion molecules, is the integrin alpha-2 chain. CD49b associates noncovalently with CD29, the integrin beta-1 subunit, to form the heterodimer integrin a2b1 that binds laminin and collagen. It is expressed on B and T lymphocytes, NK cells, monocytes, platelets, fibroblasts and long-term cultured T cells. CD49b/CD29 mediates the Mg++ dependent adhesion of platelets to collagen. It may also be utilized by fibroblasts for the reorganization of the collagen matrix during would healing.Synonyms: CD49 antigen-like family member B, Collagen Receptor, GPIa, ITGA-2, Integrin alpha-2, Platelet membrane glycoprotein Ia, VLA-2 alpha, VLA2

Gen-ID: 3673

NCBI Accession: NP_002194

UniProt: P17301

Pathways: CXCR4-mediated Signaling Events, Smooth Muscle Cell Migration, Integrin Complex